metadata = read_tsv(here('data', 'metadata_merged.tsv'), col_types = cols()) %>%
rename(TimePoint = Timepoint) %>%
mutate(TimePoint = str_replace(TimePoint, '^', 'T'),
condition = str_c(CornVariety, FungalStrain, TissueExtraction, sep='_')) %>%
rename(Corn_Genotype = CornVariety, Fungal_Treatment = FungalStrain, Tissue_Extraction = TissueExtraction)
get_counts = function(.) otu_table(.) %>% as.data.frame() %>% rownames_to_column('feature_id') %>% as_tibble()
get_tax = function(.) tax_table(.) %>% data.frame() %>% rownames_to_column('feature_id') %>% as_tibble()
get_qza = function(filepath){read_qza(filepath)$data %>% as.data.frame() %>% rownames_to_column('SampleID')}
change_its_ids_to_match_16S = . %>%
mutate(SampleID = str_replace(SampleID, '(.*?)-(.*)', '\\2-\\1'),
SampleID = str_replace_all(SampleID, c('-B73'='-373', '-C322'='-322')))
change_16S_ids_to_match_ITS = . %>%
mutate(SampleID = str_replace(SampleID, '(.*?)-(.*)', '\\2-\\1'),
SampleID = str_replace_all(SampleID, c('-373'='-B73', '-322'='-C322')))
ps %>% imap(~get_counts(.x) %>%
left_join(reads_df[[.y]], by='feature_id') %>%
left_join(get_tax(.x), by='feature_id') %>%
rename(all_of(get_sample_data(.x) %>% pull(SampleID, name=condition_w_rep))) %>%
write_csv(here(str_glue('output/raw_counts_and_taxonomy_{.y}.csv')))
)
$`16S`
$ITS
NA
Removed taxa not assigned to a phylum. After removing these taxa, 1
16S samples was removed due to having total counts <= 1500.
ps_phylum_filt = list('16S' = subset_taxa(ps$`16S`, !is.na(Phylum) &
!Phylum %in% c('', 'uncharacterized', 'unidentified') &
!Kingdom %in% c('d__Eukaryota')) %>%
prune_samples(sample_sums(.) >= 1500, .) %>% prune_taxa(taxa_sums(.) > 0, .),
'ITS' = subset_taxa(ps$ITS, !is.na(Phylum) &
ps_phylum_filt_tax = ps_phylum_filt %>% map(get_tax)
ps_phylum_filt_counts = ps_phylum_filt %>% map(get_counts)
ps_phylum_filt_ra = map(ps_phylum_filt, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_phylum_filt
phyloseq-class experiment-level object
otu_table() OTU Table: [ 946 taxa and 48 samples ]
sample_data() Sample Data: [ 48 samples by 10 sample variables ]
tax_table() Taxonomy Table: [ 946 taxa by 7 taxonomic ranks ]
phy_tree() Phylogenetic Tree: [ 946 tips and 919 internal nodes ]
$ITS
phyloseq-class experiment-level objectsample_data() Sample Data: [ 50 samples by 9 sample variables ]
reads = list('16S' = read_qza(here('data', '16S', 'rep-seqs-filtered.qza'))$data,
'ITS' = read_qza(here('data', 'ITS', 'rep-seqs-filtered.qza'))$data)
ps_phylum_filt %>% imap(function(ps_phylum_filt, seq_type){
ps_phylum_filt %>% get_tax() %>%
summarize(across(-feature_id, ~sum(!is.na(.x))/ n())) %>%
pivot_longer(everything(), names_to = 'Taxonomic rank', values_to = seq_type)
reduce(full_join, by='Taxonomic rank') %>%
kable_classic(full_width = F, html_font = "Times New Roman")
Fraction of ASVs classified at each rank
| Taxonomic rank |
16S |
ITS |
| Kingdom |
1.00 |
1.00 |
| Phylum |
1.00 |
1.00 |
| Class |
1.00 |
0.99 |
| Order |
1.00 |
0.93 |
| Family |
0.99 |
0.89 |
| Genus |
0.91 |
0.86 |
| Species |
0.28 |
0.73 |
ps_phylum_filt %>% imap(function(ps_phylum_filt, seq_type){
ps_phylum_filt %>% sample_sums() %>% enframe(name = 'SampleID', value = 'total_counts') %>%
left_join(metadata, by='SampleID') %>%
mutate(seq_type = seq_type)
ggplot(aes(x=condition, y=total_counts)) +
geom_boxplot(outlier.shape = NA) +
ggbeeswarm::geom_quasirandom(alpha = 0.3, width=0.2, groupOnX=TRUE) +
facet_wrap(~seq_type, ncol=1, scales = 'free') +
scale_y_continuous(labels = scales::comma) +
labs(title='Total number of counts for each sample') +
ggeasy::easy_center_title()

Rarefying to 1569 counts.
ps_phylum_filt_rarefied = ps_phylum_filt %>% map(
~rarefy_even_depth(.x, sample.size = 1569, rngseed=1100 , replace=FALSE) %>%
prune_taxa(taxa_sums(.) > 0, .))
ps_phylum_filt_rarefied
phyloseq-class experiment-level object
$ITS
phyloseq-class experiment-level object
otu_table() OTU Table: [ 111 taxa and 50 samples ]
sample_data() Sample Data: [ 50 samples by 9 sample variables ]
tax_table() Taxonomy Table: [ 111 taxa by 7 taxonomic ranks ]phy_tree() Phylogenetic Tree: [ 111 tips and 109 internal nodes ]
prev = map(ps_phylum_filt_ra,
function(ps_phylum_filt_ra){
relative_counts = ps_phylum_filt_ra %>% get_counts()
tax = ps_phylum_filt_ra %>% get_tax()
prevalence = rowSums(.> 0) -1, #subtracted 1 because feature_id column is always counted
Phylum = tax$Phylum,
Genus = tax$Genus,
Species = tax$Species)})
prev_plots = prev %>% imap(function(prev, seq_type){
prev %>%
mutate(prevalence = prevalence / nsamples(ps_phylum_filt[[seq_type]])) %>%
geom_hline(yintercept = 0.05, alpha = 0.5, linetype = 2) +
geom_point(size = 2, alpha = 0.6) +
scale_x_log10() + xlab("Total Relative Abundance") + ylab("Prevalence [Fraction Samples]") +
facet_wrap(~Phylum) +
labs(title = glue('{seq_type} ASV Prevalence')) +
theme(plot.title = element_text(hjust = 0.5)) +
prev_plots$`16S` %>% plotly::ggplotly()
Warning: `gather_()` was deprecated in tidyr 1.2.0.
Please use `gather()` instead.
prev_plots$ITS %>% plotly::ggplotly()
Predominant phyla
ps_phyla = map(ps_phylum_filt,
function(ps_phylum_filt){
prevalenceThreshold = 0.05 * nsamples(ps_phyla)
get_counts() %>%
prevalence = rowSums(.> 0),
TotalAbundance = taxa_sums(ps_phyla)) %>%
pull(feature_id) %>%
prune_taxa(., ps_phyla) %>%
prune_samples(sample_sums(.) >= 500, .) %>%
as('data.frame') %>%
mutate(TissueType = fct_relevel(TissueType, 'Ovule'))
return(ps_phyla)})
Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.
prev_corn_phyla = map(ps_phyla_ra,
function(ps_phyla_ra){
relative_counts = ps_phyla_ra %>% get_counts()
tax = ps_phyla_ra %>% get_tax()
relative_counts %>%
left_join(metadata, by='SampleID') %>%
left_join(tax, by='feature_id') %>%
group_by(Phylum) %>%
mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
mean_relative_abundance_entire_dataset = mean(counts)) %>%
group_by(Phylum, Corn_Genotype) %>%
mean_relative_abundance = mean(counts),
ungroup()})
prev_corn_phyla$`16S` %>%
distinct(Phylum, Corn_Genotype, mean_relative_abundance, total) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
arrange(desc(total)) %>%
kbl(format = 'html', col.names = c('Phylum', 'Total Relative Abundance',
kable_classic(full_width = F, html_font = "Times New Roman")
| Phylum |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Proteobacteria |
0.866 |
0.829 |
0.899 |
| Firmicutes |
0.065 |
0.118 |
0.016 |
| Actinobacteriota |
0.038 |
0.041 |
0.035 |
| Bacteroidota |
0.030 |
0.010 |
0.050 |
prev_corn_phyla$ITS %>%
rename(total = mean_relative_abundance_entire_dataset) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
total = round(total, 3)) %>%
pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
#filter(total >= 0.01) %>%
arrange(desc(total)) %>%
kbl(format = 'html', col.names = c('Phylum', 'Total Relative Abundance',
kable_classic(full_width = F, html_font = "Times New Roman")
| Phylum |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Ascomycota |
0.999 |
0.999 |
0.999 |
| Basidiomycota |
0.001 |
0.001 |
0.001 |
Removed taxa that are present in less than 5% of samples
for ASV level dataset. This will be used for differential abundance
testing at ASV level.
ps_prevf = map2(ps_phylum_filt, prev,
function(ps_phylum_filt, prev){
prevalenceThreshold = 0.05 * nsamples(ps_phylum_filt)
keepTaxa = filter(prev, prevalence >= prevalenceThreshold) %>%
prune_taxa(keepTaxa, ps_phylum_filt) %>%
prune_samples(sample_sums(.) >= 500, .) %>%
ps_prevf_ra = map(ps_prevf, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_prevf_clr = map(ps_prevf, microbiome::transform, 'clr')
ps_prevf_alr = map(ps_prevf, microbiome::transform, 'alr', shift=1)
ps_prevf
$`16S`
otu_table() OTU Table: [ 287 taxa and 48 samples ]
sample_data() Sample Data: [ 48 samples by 10 sample variables ]
tax_table() Taxonomy Table: [ 287 taxa by 7 taxonomic ranks ]
phy_tree() Phylogenetic Tree: [ 287 tips and 278 internal nodes ]
$ITS
phyloseq-class experiment-level objectotu_table() OTU Table: [ 51 taxa and 50 samples ]
Agglomerated counts at both genus level and species
level.
ps_genus = map(ps_phylum_filt,
ps_genus = tax_glom(ps_phylum_filt, "Genus", NArm = TRUE)
prevalenceThreshold = 0.05 * nsamples(ps_genus)
ps_genus = ps_genus %>%
get_counts() %>%
summarise(feature_id = feature_id,
prevalence = rowSums(.> 0),
TotalAbundance = taxa_sums(ps_genus)) %>%
filter(prevalence >= prevalenceThreshold) %>%
prune_taxa(., ps_genus) %>%
prune_samples(sample_sums(.) >= 500, .) %>%
prune_taxa(taxa_sums(.) > 0, .)
sample_data(ps_genus) = sample_data(ps_genus) %>%
as('data.frame') %>%
mutate(TissueType = fct_relevel(TissueType, 'Ovule'))
return(ps_genus)})
Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.
ps_genus_ra = map(ps_genus, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_genus_clr = map(ps_genus, microbiome::transform, 'clr')
ps_genus_alr = map(ps_genus, microbiome::transform, 'alr', shift=1)
ps_species = map(ps_phylum_filt,
function(ps_phylum_filt){
ps_species = ps_species %>%
get_counts() %>%
summarise(feature_id = feature_id,
TotalAbundance = taxa_sums(ps_species)) %>%
filter(prevalence >= prevalenceThreshold) %>%
pull(feature_id) %>%
prune_taxa(., ps_species) %>%
prune_samples(sample_sums(.) >= 500, .) %>%
sample_data(ps_species) = sample_data(ps_species) %>%
as('data.frame') %>%
return(ps_species)})
Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.Warning: Returning more (or less) than 1 row per `summarise()` group was deprecated in dplyr 1.1.0.
Please use `reframe()` instead.
When switching from `summarise()` to `reframe()`, remember that `reframe()` always returns an ungrouped data frame and adjust accordingly.
ps_species_ra = map(ps_species, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_species_clr = map(ps_species, microbiome::transform, 'clr')
ps_species_alr = map(ps_species, microbiome::transform, 'alr', shift=1)
Most prevalent genera
prev_corn_genus = map(ps_genus_ra,
function(ps_genus_ra){
relative_counts = ps_genus_ra %>% get_counts()
tax = ps_genus_ra %>% get_tax()
pivot_longer(-feature_id, names_to = 'SampleID', values_to = 'counts') %>%
left_join(metadata, by='SampleID') %>%
left_join(tax, by='feature_id') %>%
group_by(Genus) %>%
mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
summarize(prevalence = sum(counts > 0) / n(),
mean_relative_abundance_entire_dataset = first(mean_relative_abundance_entire_dataset)) %>%
ungroup()})
prev_corn_genus$`16S` %>%
rename(total = mean_relative_abundance_entire_dataset) %>%
distinct(Genus, Corn_Genotype, mean_relative_abundance, total) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
total = round(total, 3)) %>%
pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
filter(total >= 0.01) %>%
arrange(desc(total)) %>%
kable_classic(full_width = F, html_font = "Times New Roman")
| Genus |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Pantoea |
0.461 |
0.419 |
0.500 |
| Klebsiella |
0.094 |
0.024 |
0.159 |
| Enterobacter |
0.051 |
0.101 |
0.005 |
| Carnimonas |
0.041 |
0.085 |
0.000 |
| Burkholderia-Caballeronia-Paraburkholderia |
0.030 |
0.048 |
0.013 |
| Serratia |
0.029 |
0.033 |
0.026 |
| Stenotrophomonas |
0.029 |
0.014 |
0.043 |
| Lactococcus |
0.024 |
0.048 |
0.003 |
| Sphingobacterium |
0.024 |
0.007 |
0.039 |
| Achromobacter |
0.023 |
0.008 |
0.036 |
| Rosenbergiella |
0.022 |
0.047 |
0.000 |
| Listeria |
0.019 |
0.040 |
0.000 |
| Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium |
0.017 |
0.009 |
0.023 |
| Ochrobactrum |
0.017 |
0.013 |
0.020 |
| Enterococcus |
0.010 |
0.013 |
0.007 |
prev_corn_genus$ITS %>%
rename(total = mean_relative_abundance_entire_dataset) %>%
distinct(Genus, Corn_Genotype, mean_relative_abundance, total) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
total = round(total, 3)) %>%
arrange(desc(total)) %>%
'Relative abundance B73', 'Relative abundance CML322')) %>%
| Genus |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Aspergillus |
0.552 |
0.497 |
0.608 |
| Sarocladium |
0.252 |
0.226 |
0.279 |
| Meyerozyma |
0.124 |
0.148 |
0.101 |
| Talaromyces |
0.040 |
0.076 |
0.003 |
| Trichoderma |
0.013 |
0.026 |
0.001 |
| Penicillium |
0.006 |
0.011 |
0.001 |
| Curvularia |
0.005 |
0.007 |
0.003 |
| Alternaria |
0.004 |
0.007 |
0.002 |
| Ramichloridium |
0.001 |
0.001 |
0.001 |
| Acremonium |
0.000 |
0.001 |
0.000 |
| Bipolaris |
0.000 |
0.000 |
0.000 |
| Candida |
0.000 |
0.000 |
0.000 |
| Ceriporia |
0.000 |
0.000 |
0.000 |
| Cladosporium |
0.000 |
0.000 |
0.000 |
| Cyphellophora |
0.000 |
0.000 |
0.000 |
| Exserohilum |
0.000 |
0.000 |
0.000 |
| Fomes |
0.000 |
0.000 |
0.000 |
| Fusarium |
0.000 |
0.000 |
0.000 |
| Lecanicillium |
0.000 |
0.000 |
0.000 |
| Moesziomyces |
0.000 |
0.000 |
0.000 |
| Myrothecium |
0.000 |
0.001 |
0.000 |
| Paraconiothyrium |
0.000 |
0.000 |
0.000 |
| Peniophora |
0.000 |
0.000 |
0.000 |
| Peziza |
0.000 |
0.001 |
0.000 |
| Phanerochaete |
0.000 |
0.000 |
0.000 |
| Phlebia |
0.000 |
0.000 |
0.000 |
| Pyricularia |
0.000 |
0.000 |
0.000 |
| Scopuloides |
0.000 |
0.000 |
0.000 |
| Stereum |
0.000 |
0.001 |
0.000 |
| Tinctoporellus |
0.000 |
0.000 |
0.000 |
| Trametes |
0.000 |
0.000 |
0.000 |
| unidentified |
0.000 |
0.000 |
0.000 |
Most prevalent species
prev_corn_species = map(ps_species_ra,
function(ps_species_ra){
pivot_longer(-feature_id, names_to = 'SampleID', values_to = 'counts') %>%
left_join(metadata, by='SampleID') %>%
left_join(tax, by='feature_id') %>%
group_by(Species) %>%
mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
group_by(Species, Corn_Genotype) %>%
prevalence_entire_dataset = first(prevalence_entire_dataset),
mean_relative_abundance_entire_dataset = first(mean_relative_abundance_entire_dataset)) %>%
ungroup()})
prev_corn_species$`16S` %>%
rename(total = mean_relative_abundance_entire_dataset) %>%
distinct(Species, Corn_Genotype, mean_relative_abundance, total) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
total = round(total, 3)) %>%
pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
arrange(desc(total)) %>%
kable_classic(full_width = F, html_font = "Times New Roman")
| Species |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Pantoea_ananatis |
0.193 |
0.207 |
0.173 |
| Burkholderia_gladioli |
0.152 |
0.195 |
0.091 |
| Listeria_grayi |
0.070 |
0.119 |
0.000 |
| Lactococcus_lactis |
0.068 |
0.077 |
0.056 |
| Sphingobacterium_siyangense |
0.050 |
0.001 |
0.120 |
| Lactococcus_garvieae |
0.043 |
0.073 |
0.000 |
| Sphingobacterium_thalpophilum |
0.032 |
0.000 |
0.079 |
| Sphingomonas_phyllosphaerae |
0.030 |
0.000 |
0.073 |
| Acinetobacter_baumannii |
0.029 |
0.000 |
0.070 |
| Pseudomonas_psychrotolerans |
0.026 |
0.008 |
0.052 |
| Comamonas_sediminis |
0.023 |
0.000 |
0.056 |
| Corynebacterium_kroppenstedtii |
0.021 |
0.036 |
0.000 |
| Flavobacterium_anatoliense |
0.019 |
0.000 |
0.047 |
| Sphingobacterium_multivorum |
0.015 |
0.012 |
0.019 |
| Staphylococcus_sciuri |
0.015 |
0.026 |
0.000 |
| Devosia_riboflavina |
0.014 |
0.000 |
0.035 |
| uncultured_Tistrella |
0.013 |
0.023 |
0.000 |
| uncultured_bacterium |
0.010 |
0.014 |
0.003 |
prev_corn_species$ITS %>%
distinct(Species, Corn_Genotype, mean_relative_abundance, total) %>%
mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
total = round(total, 3)) %>%
pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
filter(total >= 0.01) %>%
arrange(desc(total)) %>%
kbl(format = 'html', col.names = c('Species', 'Total Relative Abundance',
'Relative abundance B73', 'Relative abundance CML322')) %>%
kable_classic(full_width = F, html_font = "Times New Roman")
| Species |
Total Relative Abundance |
Relative abundance B73 |
Relative abundance CML322 |
| Aspergillus_flavus |
0.567 |
0.525 |
0.608 |
| Sarocladium_zeae |
0.259 |
0.234 |
0.284 |
| Meyerozyma_caribbica |
0.135 |
0.168 |
0.102 |
| Aspergillus_niger |
0.011 |
0.021 |
0.000 |
| Talaromyces_purpureogenus |
0.010 |
0.019 |
0.000 |
Below are barplots of relative taxon abundances for 16S sequencing
with samples grouped according to similarity using the neatmap
method.
## https://github.com/google/palette.js/blob/79a703df344e3b24380ce1a211a2df7f2d90ca22/palette.js#L802
mpn65 = c('#ff0029','#377eb8','#66a61e','#984ea3','#00d2d5','#ff7f00','#af8d00','#7f80cd','#b3e900','#c42e60','#a65628',
'#f781bf','#8dd3c7','#bebada','#fb8072','#80b1d3','#fdb462','#fccde5','#bc80bd','#ffed6f','#c4eaff','#cf8c00',
'#f2b94f','#eff26e','#e43872','#d9b100','#9d7a00','#698cff','#d9d9d9','#00d27e','#d06800','#009f82','#c49200',
'#cbe8ff','#fecddf','#c27eb6','#8cd2ce','#c4b8d9','#f883b0','#a49100','#f48800','#27d0df','#a04a9b')
map(rank_names(ps_genus$`16S`)[2:6], function(tax_rank){
df = ps_genus$`16S` %>%
speedyseq::mutate_sample_data(condition = condition_w_rep) %>%
transform(transform = "compositional") %>%
p = plot_composition(df, x.label='condition', otu.sort = 'abundance', sample.sort='neatmap') +
guides(fill = guide_legend(ncol = 1)) +
scale_fill_manual(values=mpn65) +
theme_minimal() +
theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5)) +
labs(x = "Sample condition",
fill = tax_rank)
})
[[1]]
[[2]]
[[3]]
[[4]]
[[5]]
NA
The next two sets are done with ITS counts
but made the same way as above.
map(rank_names(ps_genus$ITS)[2:6], function(tax_rank){
df = ps_genus$ITS %>%
speedyseq::mutate_sample_data(condition = condition_w_rep) %>%
transform(transform = "compositional") %>%
p = plot_composition(df, x.label='condition', otu.sort = 'abundance', sample.sort='neatmap') +
guides(fill = guide_legend(ncol = 1)) +
scale_fill_manual(values=mpn65) +
theme_minimal() +
labs(x = "Sample condition",
y = "Relative abundance",
title = glue("ITS Relative abundance at {tax_rank} level"),
fill = tax_rank)
p %>% plotly::ggplotly()
})
Warning: stress is (nearly) zero: you may have insufficient data
[[1]]
[[2]]
[[3]]
[[4]]
[[5]]
NA
prune_taxa(names(sort(taxa_sums(ps_genus_ra$`16S`),decreasing = TRUE)[1:30]), ps_genus_ra$`16S`) %>%
speedyseq::plot_heatmap(method = "NMDS", distance = "bray", sample.label = 'condition', taxa.label='Genus') +
theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5, size = 10),
strip.text.x = element_text(size = 16), plot.title = element_text(size=22)) +
labs(title = '16S Heatmap of top 30 most abundant genera')

prune_taxa(names(sort(taxa_sums(ps_genus_ra$ITS),decreasing = TRUE)[1:30]), ps_genus_ra$ITS) %>%
speedyseq::plot_heatmap(method = "NMDS", distance = "bray", sample.label = 'condition', taxa.label='Genus') +
theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5, size = 10),
strip.text.x = element_text(size = 16), plot.title = element_text(size=22)) +
labs(title = 'ITS Heatmap of top 30 most abundant genera')

save.image(here('src/16_and_ITS_import.RData'))
---
title: "16S and ITS data import"
output:
  html_notebook:
    df_print: paged
    code_folding: hide
  editor_options: 
    chunk_output_type: inline
---

```{r include=FALSE}
library(qiime2R)
library(ggeasy)
library(phyloseq)
library(Biostrings)
library(ggsignif)
library(ggeasy)
library(ggpubr)
library(readxl)
library(patchwork)
library(DESeq2)
library(ALDEx2)
library(microbiome) 
library(vegan)
library(ANCOMBC)
library(glue)
library(ggstatsplot)
library(beeswarm)
library(msa)
library(here)
library(ggtext)
library(kableExtra)
library(tidyHeatmap)
library(SpiecEasi)
library(NetCoMi)
library(igraph)
library(tidygraph)
library(ggraph)
library(tidyverse)
knitr::opts_chunk$set(fig.retina = 1, dpi=450)
options(dplyr.summarise.inform=F)
theme_set(theme_bw())
sessionInfo() %>%
  capture.output(sessionInfo()) %>%
  write_lines(here('output/session_info.txt'))
```

```{r, message = FALSE, warning = FALSE}
metadata = read_tsv(here('data', 'metadata_merged.tsv'), col_types = cols()) %>%
  rename(TimePoint = Timepoint) %>%
  mutate(TimePoint = str_replace(TimePoint, '^', 'T'),
         condition = str_c(CornVariety, FungalStrain, TissueExtraction, sep='_')) %>%
  rename(Corn_Genotype = CornVariety, Fungal_Treatment = FungalStrain, Tissue_Extraction = TissueExtraction)
```

```{r}
get_counts = function(.) otu_table(.) %>% as.data.frame() %>% rownames_to_column('feature_id') %>% as_tibble()
get_tax = function(.) tax_table(.) %>% data.frame() %>% rownames_to_column('feature_id') %>% as_tibble()
get_qza = function(filepath){read_qza(filepath)$data %>% as.data.frame() %>% rownames_to_column('SampleID')}
get_sample_data = function(.) data.frame(sample_data(.)) %>% rownames_to_column('SampleID') %>% as_tibble()
change_its_ids_to_match_16S = . %>% 
  mutate(SampleID = str_replace(SampleID, '(.*?)-(.*)', '\\2-\\1'),
         SampleID = str_replace_all(SampleID, c('-B73'='-373', '-C322'='-322')))
change_16S_ids_to_match_ITS = . %>% 
  mutate(SampleID = str_replace(SampleID, '(.*?)-(.*)', '\\2-\\1'),
         SampleID = str_replace_all(SampleID, c('-373'='-B73', '-322'='-C322')))
```

```{r}
ps = list('16S'= qza_to_phyloseq(features = here('data', '16S', 'table_wo_outliers.qza'),
                          tree=here('data', '16S', 'rooted-tree.qza'),
                          taxonomy=here('data', '16S', 'merged-taxonomy.qza'),
                          metadata=here('data', '16S', 'metadata_filtered.tsv'),
                          tmp='C:/Users/brian.mack/Downloads/tmp') %>% 
            subset_samples(CornVariety != 'dummy' & Timepoint == '2' & TissueType == 'Ovule') %>%
            prune_taxa(taxa_sums(.) > 0, .),
          'ITS' = qza_to_phyloseq(features = here('data', 'ITS', 'table-no-mitochondria-no-chloroplast.qza'),
                          tree=here('data', 'ITS', 'rooted-tree.qza'),
                          taxonomy=here('data', 'ITS', 'merged-taxonomy.qza'),
                          metadata=here('data', 'ITS', 'metadata_merged.tsv'),
                          tmp='C:/Users/brian.mack/Downloads/tmp')  %>% 
            subset_samples(CornVariety  != 'dummy' & Timepoint == '2' & TissueType == 'Ovule') %>%
            prune_taxa(taxa_sums(.) > 0, .)
            )
ps = map(ps, function(.x){
  sample_data(.x) = get_sample_data(.x) %>% 
    rename(TimePoint = Timepoint) %>%
    mutate(TimePoint = str_replace(TimePoint, '^', 'T'),
           condition = str_c(CornVariety, FungalStrain, TissueExtraction, sep='_')) %>%
    rename(Corn_Genotype = CornVariety, Fungal_Treatment = FungalStrain, Tissue_Extraction = TissueExtraction) %>%
    group_by(condition) %>%
    mutate(biological_rep = row_number()) %>%
    ungroup() %>%
    mutate(condition_w_rep = str_c(condition, biological_rep, sep='_')) %>%
    column_to_rownames('SampleID')
  return(.x)})
sample_data(ps$ITS) = get_sample_data(ps$ITS) %>%
  change_its_ids_to_match_16S() %>%
  column_to_rownames('SampleID')
reads_df = reads %>% map(~tibble(sequence = as.character(.x), feature_id = names(.x)))
ps %>% imap(~get_counts(.x) %>% 
              left_join(reads_df[[.y]], by='feature_id') %>%
              left_join(get_tax(.x), by='feature_id') %>%
              rename(all_of(get_sample_data(.x) %>% pull(SampleID, name=condition_w_rep))) %>%
              write_csv(here(str_glue('output/raw_counts_and_taxonomy_{.y}.csv')))
              )
```

Removed taxa not assigned to a phylum. After removing these taxa, 1 16S samples was removed due to having total counts <= 1500.

```{r}
ps_phylum_filt = list('16S' = subset_taxa(ps$`16S`, !is.na(Phylum) & 
                                            !Phylum %in% c('', 'uncharacterized', 'unidentified') & 
                                            !Kingdom %in% c('d__Eukaryota')) %>% 
                        prune_samples(sample_sums(.) >= 1500, .) %>% prune_taxa(taxa_sums(.) > 0, .),
                      'ITS' = subset_taxa(ps$ITS, !is.na(Phylum) & 
                               !Phylum %in% c("", 'uncharacterized', 'unidentified')) %>% 
                        prune_samples(sample_sums(.) >= 1500, .) %>% prune_taxa(taxa_sums(.) > 0, .))
ps_phylum_filt_tax = ps_phylum_filt %>% map(get_tax)
ps_phylum_filt_counts = ps_phylum_filt %>% map(get_counts)
ps_phylum_filt_ra = map(ps_phylum_filt, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_phylum_filt
```
```{r}
reads = list('16S' = read_qza(here('data', '16S', 'rep-seqs-filtered.qza'))$data,
             'ITS' = read_qza(here('data', 'ITS', 'rep-seqs-filtered.qza'))$data)
```

```{r}
ps_phylum_filt %>% imap(function(ps_phylum_filt, seq_type){
  ps_phylum_filt %>% get_tax() %>%
    summarize(across(-feature_id, ~sum(!is.na(.x))/ n())) %>%
    round(2) %>%
    pivot_longer(everything(), names_to = 'Taxonomic rank', values_to = seq_type)
  }) %>%
  reduce(full_join, by='Taxonomic rank') %>%
  kbl(format = 'html', caption='Fraction of ASVs classified at each rank') %>%
  kable_classic(full_width = F, html_font = "Times New Roman") 
```

```{r, fig.height=6}
ps_phylum_filt %>% imap(function(ps_phylum_filt, seq_type){
  ps_phylum_filt %>% sample_sums() %>% enframe(name = 'SampleID', value = 'total_counts') %>%
    left_join(metadata, by='SampleID') %>%
    mutate(seq_type = seq_type)
  }) %>%
  bind_rows() %>%
  ggplot(aes(x=condition, y=total_counts)) +
  geom_boxplot(outlier.shape = NA) +
  ggbeeswarm::geom_quasirandom(alpha = 0.3, width=0.2, groupOnX=TRUE) +
  facet_wrap(~seq_type, ncol=1, scales = 'free') +
  scale_y_continuous(labels = scales::comma) +
  labs(title='Total number of counts for each sample') +
  ggeasy::easy_rotate_x_labels() +
  ggeasy::easy_center_title()
```

<br><br><br>

Rarefying to 1569 counts.

```{r, warning=FALSE, message=FALSE}
ps_phylum_filt_rarefied =  ps_phylum_filt %>% map(
  ~rarefy_even_depth(.x, sample.size = 1569, rngseed=1100 , replace=FALSE) %>%
     prune_taxa(taxa_sums(.) > 0, .))
ps_phylum_filt_rarefied
```

<br><br><br><br><br><br>

```{r, fig.width=12}
prev = map(ps_phylum_filt_ra,
            function(ps_phylum_filt_ra){
              relative_counts = ps_phylum_filt_ra %>% get_counts()
              tax = ps_phylum_filt_ra %>% get_tax()
              relative_counts %>% 
                reframe(feature_id = feature_id,
                         prevalence = rowSums(.> 0) -1, #subtracted 1 because feature_id column is always counted
                         total_relative_abundance = taxa_sums(ps_phylum_filt_ra), 
                         Phylum = tax$Phylum,
                         Genus = tax$Genus,
                         Species = tax$Species)})
prev_plots = prev %>% imap(function(prev, seq_type){
  prev %>% 
    mutate(prevalence = prevalence / nsamples(ps_phylum_filt[[seq_type]])) %>%
  ggplot(aes(total_relative_abundance, prevalence, color=Phylum, text=glue('Genus: {Genus}<br>Species: {Species}'))) +
    geom_hline(yintercept = 0.05, alpha = 0.5, linetype = 2) + 
    geom_point(size = 2, alpha = 0.6) +
    scale_x_log10() + xlab("Total Relative Abundance") + ylab("Prevalence [Fraction Samples]") +
    facet_wrap(~Phylum) +
    labs(title = glue('{seq_type} ASV Prevalence')) +
    theme_gray() +
    theme(plot.title = element_text(hjust = 0.5)) +
    theme(legend.position="none")})
```

```{r, out.width=12}
prev_plots$`16S` %>% plotly::ggplotly()
```
```{r, fig.width=12, fig.height=4}
prev_plots$ITS %>% plotly::ggplotly()
```
Predominant phyla
```{r}
ps_phyla = map(ps_phylum_filt, 
               function(ps_phylum_filt){
                 ps_phyla = tax_glom(ps_phylum_filt, "Phylum", NArm = TRUE)
                 prevalenceThreshold =  0.05 * nsamples(ps_phyla)
                 ps_phyla = ps_phyla %>% 
                   get_counts() %>% 
                   summarise(feature_id = feature_id,
                             prevalence = rowSums(.> 0),
                             TotalAbundance = taxa_sums(ps_phyla)) %>%
                   filter(prevalence >= prevalenceThreshold) %>% 
                   pull(feature_id) %>%
                   prune_taxa(., ps_phyla) %>%
                   prune_samples(sample_sums(.) >= 500, .) %>% 
                   prune_taxa(taxa_sums(.) > 0, .)
                 sample_data(ps_phyla) = sample_data(ps_phyla) %>% 
                   as('data.frame') %>%
                   mutate(TissueType = fct_relevel(TissueType, 'Ovule'))
                 return(ps_phyla)})
ps_phyla_ra = map(ps_phyla, ~transform_sample_counts(.x, function(x){x / sum(x)}))
prev_corn_phyla = map(ps_phyla_ra,
            function(ps_phyla_ra){
            relative_counts = ps_phyla_ra %>% get_counts()
              tax = ps_phyla_ra %>% get_tax()
              relative_counts %>% 
                pivot_longer(-feature_id, names_to = 'SampleID', values_to = 'counts') %>%
                left_join(metadata, by='SampleID') %>%
                left_join(tax, by='feature_id') %>%
                group_by(Phylum) %>%
                mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
                       mean_relative_abundance_entire_dataset = mean(counts)) %>%
                group_by(Phylum, Corn_Genotype) %>%
                summarize(prevalence = sum(counts > 0) / n(),
                          mean_relative_abundance = mean(counts),
                          prevalence_entire_dataset = first(prevalence_entire_dataset),
                          mean_relative_abundance_entire_dataset = first(mean_relative_abundance_entire_dataset)) %>%
                ungroup()})
prev_corn_phyla$`16S` %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Phylum, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Phylum', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
prev_corn_phyla$ITS %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Phylum, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  #filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Phylum', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
```
<br><br><br> Removed taxa that are present in less than 5% of samples for ASV level dataset. This will be used for differential abundance testing at ASV level.
```{r}
ps_prevf = map2(ps_phylum_filt, prev,
               function(ps_phylum_filt, prev){
                 prevalenceThreshold =  0.05 * nsamples(ps_phylum_filt)
                 keepTaxa = filter(prev, prevalence >= prevalenceThreshold) %>% 
                   pull(feature_id)
                 prune_taxa(keepTaxa, ps_phylum_filt) %>%
                   prune_samples(sample_sums(.) >= 500, .) %>% 
                   prune_taxa(taxa_sums(.) > 0, .)})
ps_prevf_ra = map(ps_prevf, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_prevf_clr = map(ps_prevf, microbiome::transform, 'clr')
ps_prevf_alr = map(ps_prevf, microbiome::transform, 'alr', shift=1)
ps_prevf
```

<br><br><br> Agglomerated counts at both genus level and species level.

```{r}
ps_genus = map(ps_phylum_filt, 
               function(ps_phylum_filt){
                 ps_genus = tax_glom(ps_phylum_filt, "Genus", NArm = TRUE)
                 prevalenceThreshold =  0.05 * nsamples(ps_genus)
                 ps_genus = ps_genus %>% 
                   get_counts() %>% 
                   summarise(feature_id = feature_id,
                             prevalence = rowSums(.> 0),
                             TotalAbundance = taxa_sums(ps_genus)) %>%
                   filter(prevalence >= prevalenceThreshold) %>% 
                   pull(feature_id) %>%
                   prune_taxa(., ps_genus) %>%
                   prune_samples(sample_sums(.) >= 500, .) %>% 
                   prune_taxa(taxa_sums(.) > 0, .)
                 sample_data(ps_genus) = sample_data(ps_genus) %>% 
                   as('data.frame') %>%
                   mutate(TissueType = fct_relevel(TissueType, 'Ovule'))
                 return(ps_genus)})
ps_genus_ra = map(ps_genus, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_genus_clr = map(ps_genus, microbiome::transform, 'clr')
ps_genus_alr = map(ps_genus, microbiome::transform, 'alr', shift=1)
ps_species = map(ps_phylum_filt, 
               function(ps_phylum_filt){
                 ps_species = tax_glom(ps_phylum_filt, "Species", NArm = TRUE)
                 prevalenceThreshold =  0.05 * nsamples(ps_species)
                 ps_species = ps_species %>% 
                   get_counts() %>% 
                   summarise(feature_id = feature_id,
                             prevalence = rowSums(.> 0),
                             TotalAbundance = taxa_sums(ps_species)) %>%
                   filter(prevalence >= prevalenceThreshold) %>% 
                   pull(feature_id) %>%
                   prune_taxa(., ps_species) %>%
                   prune_samples(sample_sums(.) >= 500, .) %>% 
                   prune_taxa(taxa_sums(.) > 0, .)
                 sample_data(ps_species) = sample_data(ps_species) %>% 
                   as('data.frame') %>%
                   mutate(TissueType = fct_relevel(TissueType, 'Ovule'))
                 return(ps_species)})
ps_species_ra = map(ps_species, ~transform_sample_counts(.x, function(x){x / sum(x)}))
ps_species_clr = map(ps_species, microbiome::transform, 'clr')
ps_species_alr = map(ps_species, microbiome::transform, 'alr', shift=1)
```

Most prevalent genera
```{r}
prev_corn_genus = map(ps_genus_ra,
            function(ps_genus_ra){
            relative_counts = ps_genus_ra %>% get_counts()
              tax = ps_genus_ra %>% get_tax()
              relative_counts %>% 
                pivot_longer(-feature_id, names_to = 'SampleID', values_to = 'counts') %>%
                left_join(metadata, by='SampleID') %>%
                left_join(tax, by='feature_id') %>%
                group_by(Genus) %>%
                mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
                       mean_relative_abundance_entire_dataset = mean(counts)) %>%
                group_by(Genus, Corn_Genotype) %>%
                summarize(prevalence = sum(counts > 0) / n(),
                          mean_relative_abundance = mean(counts),
                          prevalence_entire_dataset = first(prevalence_entire_dataset),
                          mean_relative_abundance_entire_dataset = first(mean_relative_abundance_entire_dataset)) %>%
                ungroup()})
prev_corn_genus$`16S` %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Genus, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Genus', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
prev_corn_genus$ITS %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Genus, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  #filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Genus', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
```
Most prevalent species
```{r}
prev_corn_species = map(ps_species_ra,
            function(ps_species_ra){
            relative_counts = ps_species_ra %>% get_counts()
              tax = ps_species_ra %>% get_tax()
              relative_counts %>% 
                pivot_longer(-feature_id, names_to = 'SampleID', values_to = 'counts') %>%
                left_join(metadata, by='SampleID') %>%
                left_join(tax, by='feature_id') %>%
                group_by(Species) %>%
                mutate(prevalence_entire_dataset = sum(counts > 0) / n(),
                       mean_relative_abundance_entire_dataset = mean(counts)) %>%
                group_by(Species, Corn_Genotype) %>%
                summarize(prevalence = sum(counts > 0) / n(),
                          mean_relative_abundance = mean(counts),
                          prevalence_entire_dataset = first(prevalence_entire_dataset),
                          mean_relative_abundance_entire_dataset = first(mean_relative_abundance_entire_dataset)) %>%
                ungroup()})
prev_corn_species$`16S` %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Species, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Species', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
prev_corn_species$ITS %>% 
  rename(total = mean_relative_abundance_entire_dataset) %>%
  distinct(Species, Corn_Genotype, mean_relative_abundance, total) %>%
  mutate(mean_relative_abundance= round(mean_relative_abundance, 3),
         total = round(total, 3)) %>%
  pivot_wider(names_from = Corn_Genotype, values_from = mean_relative_abundance) %>%
  filter(total >= 0.01) %>%
  arrange(desc(total)) %>%
  kbl(format = 'html', col.names = c('Species', 'Total Relative Abundance', 
                                     'Relative abundance B73', 'Relative abundance CML322')) %>%
  kable_classic(full_width = F, html_font = "Times New Roman")
```


Below are barplots of relative taxon abundances for 16S sequencing with samples grouped according to similarity using the neatmap method. 

```{r, fig.width=12, fig.height=10, warning=FALSE, fig.show='hide'}
## https://github.com/google/palette.js/blob/79a703df344e3b24380ce1a211a2df7f2d90ca22/palette.js#L802
mpn65 = c('#ff0029','#377eb8','#66a61e','#984ea3','#00d2d5','#ff7f00','#af8d00','#7f80cd','#b3e900','#c42e60','#a65628',
         '#f781bf','#8dd3c7','#bebada','#fb8072','#80b1d3','#fdb462','#fccde5','#bc80bd','#ffed6f','#c4eaff','#cf8c00',
         '#1b9e77','#d95f02','#e7298a','#e6ab02','#a6761d','#0097ff','#00d067','#000000','#252525','#525252','#737373',
         '#969696','#bdbdbd','#f43600','#4ba93b','#5779bb','#927acc','#97ee3f','#bf3947','#9f5b00','#f48758','#8caed6',
         '#f2b94f','#eff26e','#e43872','#d9b100','#9d7a00','#698cff','#d9d9d9','#00d27e','#d06800','#009f82','#c49200',
         '#cbe8ff','#fecddf','#c27eb6','#8cd2ce','#c4b8d9','#f883b0','#a49100','#f48800','#27d0df','#a04a9b')
```

```{r, fig.width=12, fig.height=8}
map(rank_names(ps_genus$`16S`)[2:6], function(tax_rank){
  df = ps_genus$`16S` %>% 
    speedyseq::mutate_sample_data(condition = condition_w_rep) %>%
    transform(transform = "compositional") %>%
    aggregate_rare(level = tax_rank, detection = 0.05, prevalence = 0.05) 
  p = plot_composition(df, x.label='condition', otu.sort = 'abundance', sample.sort='neatmap') +
  guides(fill = guide_legend(ncol = 1)) +
  scale_fill_manual(values=mpn65) +
  theme_minimal() + 
  theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5)) +
  labs(x = "Sample condition",
       y = "Relative abundance",
       title = glue("16S Relative abundance at {tax_rank} level"), 
       fill = tax_rank)
     p %>% plotly::ggplotly()
})
```


<br><br><br><br><br><br> The next two sets are done with ITS counts but made the same way as above.

```{r, fig.width=12, fig.height=8}
map(rank_names(ps_genus$ITS)[2:6], function(tax_rank){
  df = ps_genus$ITS %>% 
    speedyseq::mutate_sample_data(condition = condition_w_rep) %>%
    transform(transform = "compositional") %>%
    aggregate_rare(level = tax_rank, detection = 0.05, prevalence = 0.05) 
  p = plot_composition(df, x.label='condition', otu.sort = 'abundance', sample.sort='neatmap') +
  guides(fill = guide_legend(ncol = 1)) +
  scale_fill_manual(values=mpn65) +
  theme_minimal() + 
  theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5)) +
  labs(x = "Sample condition",
       y = "Relative abundance",
       title = glue("ITS Relative abundance at {tax_rank} level"), 
       fill = tax_rank)
  p %>% plotly::ggplotly()
})
```


```{r, fig.width=15, fig.height=8, warning=FALSE}
prune_taxa(names(sort(taxa_sums(ps_genus_ra$`16S`),decreasing = TRUE)[1:30]), ps_genus_ra$`16S`) %>%
  speedyseq::plot_heatmap(method = "NMDS", distance = "bray", sample.label = 'condition', taxa.label='Genus') +
  theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5, size = 10),
        strip.text.x = element_text(size = 16), plot.title = element_text(size=22)) +
  labs(title = '16S Heatmap of top 30 most abundant genera')
```

<br><br><br><br><br><br>

```{r, fig.width=15, fig.height=8, warning=FALSE}
prune_taxa(names(sort(taxa_sums(ps_genus_ra$ITS),decreasing = TRUE)[1:30]), ps_genus_ra$ITS) %>%
  speedyseq::plot_heatmap(method = "NMDS", distance = "bray", sample.label = 'condition', taxa.label='Genus') +
  theme(axis.text.x=element_text(angle=90,hjust=0, vjust=0.5, size = 10),
        strip.text.x = element_text(size = 16), plot.title = element_text(size=22)) +
  labs(title = 'ITS Heatmap of top 30 most abundant genera')
```
```{r}
save.image(here('src/16_and_ITS_import.RData'))
```

